Brigg’s Liver Tomato Broth

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brigg’s Liver Tomato Broth

培养基配方
Composition per liter:
Glucose .....................................................20.0g
Neopeptone...................................................15.0g
Yeast extract.................................................6.0g
NaCl..........................................................5.0g
Tween™ 80.....................................................1.0g
Soluble starch................................................0.5g
L -Cysteine·HCl..............................................0.2g
Tomato juice...............................................400.0mL
Liver extract...............................................75.0mL
pH 5.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. (Note: 3.0g of proteolysed liver may be used
instead of the liver extract.) Mix thoroughly. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Lactobacillus acetotolerans.