​BRILA MUG Broth (Brillant Green 2%-Bile MUG Broth)

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BRILA MUG Broth
(Brillant Green 2%-Bile MUG Broth)

培养基配方
Composition per liter:
Ox-bile (dried) ..........................................20.0g
Peptone...................................................10.0g
Lactose...................................................10.0g
L -Tryptophan..............................................1.0g
Brillant Green...........................................0.133g
4-Methylumbelliferyl-ß-D-glucuronide......................0.1g
pH 7.2 ± 0.2 at 37°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube in 10.0mL volumes. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use : For the detection of E. coli and coliforms. Bile and Brilliant
Green extensively inhibit the growth of accompanying flora, in partic-
ular Gram-positive microorganisms. The presence of E. coli results in
fluorescence in the UV. A positive indole test and possibly gas forma-
tion from lactose fermentation provide confirmation. β- D -glucoroni-
dase, which is produced by E. coli, cleaves 4-methylumbelliferyl-β- D -
glucuronide to 4-methylumbelliferone and glucuronide. The fluorogen
4-methylumbelliferone can be detected under a long wavelength UV
lamp.The broth can be used in conjunction with the MPN method for
E. coli and coliform enumeration in the water of bathing areas.