Brilliance™ Bacillus cereus Agar (Chromogenic Bacillus cereus Agar)

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ Bacillus cereus Agar
(Chromogenic Bacillus cereus Agar)

培养基配方
Composition per liter:
Agar..........................................................13.0g
Peptone ......................................................10.0g
Sodium pyruvate...............................................10.0g
Yeast extract ................................................4.0g
Na2HPO4 .....................................................2.52g
Chromogenic mix ..............................................1.2g
KH2PO4 ......................................................0.28g
Bacillus cereus selective supplement .......................10.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Bacillus cereus Selective Supplement:
Composition per 10.0mL:
Trimethoprim...............................................10.0mg
Polymyxin B.............................................106,000 U
Preparation of Bacillus cereus Selective Supplement Solution:
Add components to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except Bacillus cereus
selective supplement. to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally add 5.0mL Bacillus cereus selective supplement. Mix thourough-
ly. Pour into sterile Petri dishes.
Use: For the isolation and differentiation of Bacillus cereus from food
samples.