Brilliance™ Candida Agar

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ Candida Agar

培养基配方
Composition per liter:
Chromogenic mix ............................................13.6g
Agar........................................................13.6g
Peptone .....................................................4.0g
Selective supplement solution..............................10.0mL
pH 6.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Selective Supplement Solution:
Composition per 10.0mL:
Chloramphenicol.............................................0.5g
Preparation of Selective Supplement Solution:  Add chloram-
phenicol to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Add 10.0mL selective supplement solution.
Gently heat while stirring and bring to boiling. Do not autoclave. Cool
to 45°C. Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the rapid isolation and identification of clinically important
Candida species. The green color of Candida albicans and Candida
dubliniensis is caused by the same chromogenic reaction as the dark
blue color of Candida tropicalis. Candida glabrata, Candida kefyr,
Candida parapsilosis, and Candida lusitaniae appear as a variety of
beige/brown/yellow colors, due to the mixture of natural pigmentation
and some alkaline phosphatase activity.