Brilliance™ E. coli/Coliform Agar (Chromogenic E. coli/Coliform Agar)

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ E. coli/Coliform Agar
(Chromogenic E. coli/Coliform Agar)

培养基配方
Composition per liter:
Chromogenic mix ..............................................20.3g
Agar .........................................................15.0g
Peptone........................................................5.0g
NaCl...........................................................5.0g
Na2HPO4 .......................................................3.5g
Yeast extract..................................................3.0g
Lactose........................................................2.5g
KH2PO4 ........................................................1.5g
Neutral Red...................................................0.03g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the presumptive identification of Escherichia coli and coli-
forms from food and environmental samples. E. coli forms pink colo-
nies.