Brilliance™ Listeria Agar (Oxoid Chromogenic Listeria Agar) (OCLA)

[所属分类:培养基配方] [发布时间:2020-1-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ Listeria Agar
(Oxoid Chromogenic Listeria Agar)
(OCLA)

培养基配方
Composition per liter:
Peptone ....................................................18.5g
LiCl........................................................15.0g
Agar .......................................................14.0g
NaCl.........................................................9.5g
Yeast extract................................................4.0g
Maltose .....................................................4.0g
Sodium pyruvate .............................................2.0g
X-glucoside chromogenic mix .................................0.2g
Differential lecithin solution.............................40.0mL
Selective supplement solution .............................20.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Differential Lecithin Solution:
Composition per 40.0mL:
Lecithin....................................................Proprietary
Preparation of Differential Lecithin Solution:  Available as pre-
mixed solution.
Selective Supplement Solution:
Composition per 20.0mL:
Nalidixic acid.............................................26.0mg
Polymyxin B................................................10.0mg
Ceftazidime.................................................6.0mg
Amphotericin...............................................10.0mg
Preparation of Selective Supplement Solution:  Add components
to distilled/deionized water and bring volume to 20.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except differential lec-
ithin solution and selective supplement solution, to distilled/deionized
water and bring volume to 940.0mL. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 46°C. Aseptially add differential lecithin solution and
selective supplement solution. Mix thoroughly. Pour into sterile Petri
dishes.
Use:  For the isolation, enumeration, and presumptive identification of
Listeria species and Listeria monocytogenes from food samples. A
chromogenic agar for the selective growth and differentiation of Liste-
ria monocytogenes and Listeria spp.