Brilliance™ MRSA Agar

[所属分类:培养基配方] [发布时间:2020-1-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ MRSA Agar

培养基配方
Composition per liter:
Peptone mix .................................................25.0g
Salt mix ....................................................25.0g
Agar ........................................................15.0g
Kaolin.......................................................13.0g
Chromogenic mix ..............................................2.0g
Antibiotic cocktail .........................................4.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components, except antibiotic cock-
tail, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat while stirring and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptially add antibi-
otic cocktail. Mix thoroughly. Pour into sterile Petri dishes.
Use:  For universal MRSA screening. Oxoid Brilliance MRSA Agar
incorporates a novel chromogen that yields a blue color as a result of
phosphatase activity, indicative of many staphylococci including
Staphylococcus aureus. To allow the medium to differentiate MRSA
accurately, it contains a combination of antibacterial compounds
designed to inhibit the growth of a wide variety of competitor organ-
isms and MSSA. Also included are compounds to suppress the expres-
sion of phosphatase activity in other staphylococci, thus ensuring a
high level of sensitivity and specificity.