Brilliance™ Salmonella Agar

[所属分类:培养基配方] [发布时间:2020-1-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ Salmonella Agar

培养基配方
Composition per liter:
Chromogenic mix ...........................................25.0g
Agar.......................................................15.0g
Inhibigen™ mix.............................................14.0g
Salmonella selective supplement solution...................10.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Salmonella Selective Supplement Solution:
Composition per 10.0mL:
Novobiocin ................................................10.0mg
Cefsulodin.................................................24.0mg
Preparation of Selective Supplement Solution:  Add components
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except Salmonella se-
lective supplement solution, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Add 10.0mL Salmonella selective
supplement. It is critical that the selective supplement is added prior to
heating. Gently heat while stirring and bring to boiling. Do not auto-
clave. Cool to 50°C. Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the presumptice detection and identification of Salmonella
spp. from foods and clinical specimens. The Inhibigen contained in this
medium specifically targets E.coli, a particular benefit when testing
fecal samples. Additional compounds are added to suppress growth of
other competing flora. Differentiation of Salmonella from the other
organisms that grow on Brilliance Salmonella Agar is achieved
through the inclusion of two chromogens that target specific enzymes:
caprylate esterase and β-glucosidase. The action of the enzymes on the
chromogens results in a build-up of color within the colony. The color
produced depends on which enzymes the organisms possess. The
action of caprylate esterase present in all salmonellae results in a purple
colony. Some Enterobacteriaceae species also produce caprylate
esterase, but these are differentiated from Salmonella by a β-glucosi-
dase substrate. This results in blue colonies, which are easy to distin-
guish from the purple Salmonella colonies.