Brilliance™ UTI Clarity Agar

[所属分类:培养基配方] [发布时间:2020-1-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ UTI Clarity Agar

培养基配方
Composition per liter:
Chromogenic mix .........................................17.0g
Agar ....................................................10.0g
Peptone...................................................9.0g
Tryptophan................................................1.0g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the detection and differentiation of coliform bacteria. For the
presumptive identification of the main pathogens which cause infec-
tion of the urinary tract. Brilliance UTI Clarity Agar contains two chro-
mogenic substrates which are cleaved by enzymes produced by E. coli,
Enterococcus spp., and coliforms. Of the two chromogens included in
the medium, one is metabolized by β-galactosidase, an enzyme pro-
duced by E. coli, which grow as pink colonies. The other is cleaved by
β-glucosidase enzyme activity, allowing the specific detection of
enterococci which form blue or turquoise colonies. Cleavage of both
the chromogens gives dark blue or purple colonies, and indicates the
organism is a coliform. The tryptophan in the medium is an indicator
of tryptophan deaminase activity, resulting in colonies of Proteus,
Morganella, and Providencia spp. with brown halos.