Brilliant Green Agar

[所属分类:培养基配方] [发布时间:2020-1-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Agar

培养基配方
Composition per liter:
Agar .....................................................20.0g
Lactose...................................................10.0g
Sucrose...................................................10.0g
Peptic digest of animal tissue.............................5.0g
Pancreatic digest of casein................................5.0g
NaCl.......................................................5.0g
Phenol Red................................................0.08g
Brilliant Green ........................................0.0125g
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath and BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the selective isolation of Salmonella other than Salmonella
typhi from feces and other specimens, and food and dairy products.
Salmonella other than Salmonella typhi appear as red/pink/white col-
onies surrounded by a zone of red in the agar, indicating nonlactose/
sucrose fermentation. Proteus or Pseudomonas species may appear as
small red colonies. Lactose- or sucrose-fermenting bacteria appear as
yellow-green colonies surrounded by a zone of yellow-green in the
agar.