Brilliant Green Agar Base with Phosphates and Sulfa Supplement

[所属分类:培养基配方] [发布时间:2020-1-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Agar Base
with Phosphates and Sulfa Supplement

培养基配方
Composition per liter:
Agar......................................................12.0g
Sucrose...................................................10.0g
Plant peptone ............................................10.0g
Lactose...................................................10.0g
Plant extract..............................................5.0g
Yeast extract..............................................3.0g
Na2HPO4 ...................................................1.0g
NaH2PO4 ...................................................0.6g
Phenol Red................................................0.09g
Brilliant Green...........................................4.7mg
pH 6.9 ± 0.2 at 25°C
Source: This medium, without sulfa supplement, is available as a pre-
mixed powder from HiMedia.
Sulfa Supplement Solution:
Composition per 10.0mL:
Sodium sulfacetamide........................................1.0g
Sodium mandelate ..........................................0.25g
Preparation of Sulfa Supplement Solution:  Add components to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except sulfa supple-
ment solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile sulfa supplement solution.
Mix thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation of Salmonella other than Salmonella
typhi from feces and other specimens while inhibiting Escherichia
coli, Proteus, and Pseudomonas species.