Brucella Anaerobic Blood Agar

[所属分类:培养基配方] [发布时间:2020-3-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Anaerobic Blood Agar

培养基配方
Composition per liter:
Vitamin K 1 .....................................................0.01g
Anaerobic agar base...........................................1000.0mL
Sheep blood, sterile, defibrinated .............................50.0mL
Anaerobic Agar Base
Composition per liter:
Pancreatic digest of casein......................................17.5g
Agar ............................................................15.0g
Glucose .........................................................10.0g
Papaic digest of soybean meal....................................2.5g
NaCl.............................................................2.5g
Sodium thioglycolate.............................................2.0g
Sodium formaldehyde sulfoxylate..................................1.0g
L- Cystine·HCl·H2O .............................................0.4g
Methylene Blue..................................................0.002g
pH 7.0 ± 0.2 at 25°C
Preparation of Anaerobic Agar Base:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: To 950.0mL of cooled, sterile anaerobic
agar base, aseptically add 10.0mg of vitamin K 1 and 50.0mL of sterile, de-
fibrinated sheep blood.
Use: For the isolation of anaerobes.