Brucella Blood Agar with Hemin and Vitamin K 1

[所属分类:培养基配方] [发布时间:2020-3-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Blood Agar with Hemin and Vitamin K 1

培养基配方
Composition per liter:
Agar.......................................................15.0g
Pancreatic digest of casein................................10.0g
Peptic digest of animal tissue ............................10.0g
NaCl........................................................5.0g
Yeast extract...............................................2.0g
Glucose.....................................................1.0g
NaHSO3 .....................................................0.1g
Vitamin K 1 ...............................................1.0mL
Hemin .....................................................1.0mL
Sheep blood, defibrinated.................................50.0mL
Source:  This medium is available as a prepared medium from BD Di-
agnostic Systems.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................1.0g
Ethanol, absolute.............................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin .........................................................1.0g
NaOH (1N solution).............................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium:  Add components, except vitamin K 1 so-
lution and sheep blood, to distilled/deionized water and bring volume
to 949.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add 1.0mL of sterile vitamin K 1 solution and 50.0mL of sterile
defibrinated sheep blood. Mix gently and pour into sterile Petri dishes.
Use: For the isolation and cultivation of anaerobic microorganisms
from clinical and nonclinical specimens. After growth on agar plates,
colonies should be examined under a dissecting microscope under
long-wave UV light. Members of the pigmented Bacteroides group
appear as red/orange fluorescent colonies.