Brucella Blood Culture Broth

[所属分类:培养基配方] [发布时间:2020-3-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Blood Culture Broth

培养基配方
Composition per liter:
Sucrose.....................................................100.0g
Hemin ........................................................0.5g
Sodium polyanetholsulfonate (SPS)............................0.25g
Brucella broth base.......................................1000.0mL
Vitamin K 1 solution.........................................1.0mL
pH 7.0 ± 0.2 at 25°C
Brucella Broth Base:
Composition per liter:
Pancreatic digest of casein......................................10.0g
Peptic digest of animal tissue ..................................10.0g
NaCl.............................................................5.0g
Yeast extract....................................................2.0g
Glucose..........................................................1.0g
NaHSO3 .........................................................0.1g
Preparation of Brucella Broth Base: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 .........................................................1.09g
Ethanol, absolute...................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Store in the dark at 4°C.
Preparation of Medium: Add components, except vitamin K 1 so-
lution, to prepared Brucella broth base. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of vitamin
K 1 solution. Distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of microorganisms from blood.
Especially useful for the cultivation of anaerobes.