Brucella Broth with Additives (ATCC Medium 489)

[所属分类:培养基配方] [发布时间:2020-3-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Broth with Additives
(ATCC Medium 489)

培养基配方
Composition per liter:
Pancreatic digest of casein.................................10.0g
Peptic digest of animal tissue..............................10.0g
NaCl........................................................3.5g
Yeast extract...............................................2.0g
Glucose ....................................................1.0g
NaHSO3 .....................................................0.1g
Horse serum, inactivated.................................100.0mL
Fresh yeast extract solution.............................50.0mL
pH 7.0 ± 0.2 at 25°C
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free....................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8.
Preparation of Medium: Add components, except horse serum
and fresh yeast extract solution, to distilled/deionized water and bring
volume to 850.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 100.0mL of sterile horse serum and 50.0mL of sterile
fresh yeast extract solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use:  For the cultivation of Corynebacterium species.