- 定做培养基/定制培养基
- 颗粒培养基
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- 2025年版中国药典
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- 样品采集与处理(均质)产品
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- 生化反应鉴定管
- 染色液等配套产品
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- 实验耗材与器具
- 生化试剂/化学试剂
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Brucella Broth with 0.16% Agar (ATCC Medium 1116)
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Brucella Broth with 0.16% Agar
(ATCC Medium 1116)
培养基配方
Composition per liter:
Pancreatic digest of casein..............................10.0g
Peptic digest of animal tissue ..........................10.0g
NaCl......................................................5.0g
Yeast extract.............................................2.0g
Agar......................................................1.6g
Glucose...................................................1.0g
NaHSO3 ...................................................0.1g
Horse blood, defibrinated ..............................50.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium without agar is available as a premixed powder
from BD Diagnostic Systems.
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Heat gently with frequent mixing. Boil for 1 min. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
50.0mL of sterile horse blood. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation and maintenance of Campylobacter fetus
subsp. fetus and Campylobacter jejuni subsp. jejuni.