Brucella Broth Base Campylobacter Medium

[所属分类:培养基配方] [发布时间:2020-3-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Broth Base Campylobacter Medium

培养基配方
Composition per liter:
Cycloheximide (Actidione ® )..............................50.0mg
Sodium cephazolin.........................................15.0mg
Novobiocin.................................................5.0mg
Bacitracin...............................................25,000U
Colistin sulfate.........................................10,000U
Brucella broth base......................................900.0mL
Horse blood, defibrinated ...............................100.0mL
pH 7.0 ± 0.2 at 25°C
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Brucella Broth Base:
Composition per liter:
Pancreatic digest of casein......................................10.0g
Peptic digest of animal tissue...................................10.0g
NaCl.............................................................5.0g
Yeast extract....................................................2.0g
Glucose .........................................................1.0g
NaHSO3 ..........................................................0.1g
Preparation of Brucella Broth Base: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Optional Supplement:
Composition per 10.0mL:
Sodium pyruvate..............................................0.25g
NaHSO3 ......................................................0.25g
FeSO4 ·7H2O.................................................0.25g
Preparation of Optional Supplement: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Filter sterilize.
Preparation of Medium: Add components, except horse blood, to
900.0mL of prepared Brucella broth base. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 100.0mL of sterile, defibrinated horse blood. Addition of 10.0mL
of optional supplement will improve growth. Mix thoroughly. Pour
into sterile Petri dishes.
Use: For the selective isolation and cultivation of Campylobacter
jejuni from fecal specimens or rectal swabs. Addition of the optional
supplement improves growth.