Brucella Broth, Modified

[所属分类:培养基配方] [发布时间:2020-3-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Broth, Modified

培养基,培养基配方
Composition per liter:
Pancreatic digest of casein................................10.0g
Peptic digest of animal tissue.............................10.0g
NaCl........................................................5.0g
MgSO4 ·7H2O...............................................2.46g
Yeast extract..............................................2.0g
CaCl2 .....................................................1.1g
Glucose ...................................................1.0g
NaHSO3 ....................................................0.1g
Horse blood, defibrinated ...............................100.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse
blood. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation and maintenance of Campylobacter coli and
Campylobacter fecalis.