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Brucella Selective Medium with Blood and Serum

[所属分类:培养基配方] [发布时间:2020-3-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Selective Medium with Blood and Serum

培养基配方
Composition per liter:
Brain heart, infusion from....................................500.0g
Agar .........................................................15.0g
Tryptose .....................................................10.0g
Glucose ......................................................2.5g
NaCl..........................................................5.0g
Gelatin.......................................................1.0g
Sheep blood, defibrinated..................................100.0mL
Horse serum.................................................50.0mL
Vitamin K 1 solution.........................................1.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Vitamin K 1 Solution:
Composition per 20.0mL:
Vitamin K 1 .................................................0.2g
Ethanol, absolute..........................................20.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 20.0mL
of ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sheep blood,
horse serum, and vitamin K 1 solution, to distilled/deionized water and
bring volume to 849.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 100.0mL of sterile sheep blood, 50.0mL of
horse serum, and 1.0mL of sterile vitamin K 1 solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Brucella species.

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