- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
Brucella Selective Medium with Blood and Serum
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Brucella Selective Medium with Blood and Serum
培养基配方
Composition per liter:
Brain heart, infusion from....................................500.0g
Agar .........................................................15.0g
Tryptose .....................................................10.0g
Glucose ......................................................2.5g
NaCl..........................................................5.0g
Gelatin.......................................................1.0g
Sheep blood, defibrinated..................................100.0mL
Horse serum.................................................50.0mL
Vitamin K 1 solution.........................................1.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Vitamin K 1 Solution:
Composition per 20.0mL:
Vitamin K 1 .................................................0.2g
Ethanol, absolute..........................................20.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 20.0mL
of ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sheep blood,
horse serum, and vitamin K 1 solution, to distilled/deionized water and
bring volume to 849.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 100.0mL of sterile sheep blood, 50.0mL of
horse serum, and 1.0mL of sterile vitamin K 1 solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Brucella species.