Brucella Semisolid Medium with Cysteine

[所属分类:培养基配方] [发布时间:2020-3-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Semisolid Medium with Cysteine

培养基配方
Composition per liter:
Peptamin.......................................................10.0g
Pancreatic digest of casein....................................10.0g
Glycine........................................................10.0g
NaCl...........................................................5.0g
Yeast extract..................................................2.0g
Agar...........................................................1.8g
Glucose........................................................1.0g
L -Cysteine·HCl·H2O..........................................0.2g
NaHSO3 ........................................................0.1g
Sodium citrate.................................................0.1g
Neutral Red solution.........................................10.0mL
pH 7.0 ± 0.2 at 25°C
Neutral Red Solution:
Composition per 100.0mL:
Neutral Red....................................................0.2g
Ethanol......................................................10.0mL
Preparation of Neutral Red Solution: Add Neutral Red to
10.0mL of ethanol. Bring volume to 100.0mL.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of Campylobacter species
based on H2S production from cysteine.