BSK Medium (Barbour-Stoenner-Kelly Medium)

[所属分类:培养基配方] [发布时间:2020-3-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BSK Medium
(Barbour-Stoenner-Kelly Medium)

培养基配方
Composition per 1260.0mL:
Bovine albumin fraction V ...................................50.0g
HEPES (N-[2-hydroxyethyl]piperazine-N′-2-
ethanesulfonic acid) buffer...................................6.0g
Neopeptone....................................................5.0g
Glucose ......................................................5.0g
NaHCO3 .......................................................2.2g
Sodium pyruvate...............................................0.8g
Sodium citrate................................................0.7g
N-Acetylglucosamine...........................................0.4g
Gelatin solution...........................................200.0mL
CMRL 1066, without glutamine,
without bicarbonate, 10X...................................100.0mL
Rabbit serum................................................72.0mL
pH 7.6–7.65 at 25°C
Gelatin Solution:
Composition per 200.0mL:
Gelatin......................................................14.0g
Preparation of Gelatin Solution: Add gelatin to distilled/deion-
ized water and bring volume to 200.0mL. Heat gently to boiling. Mix
thoroughly. Filter sterilize.

CMRL 1066 Medium without Glutamine, without Bicar-
bonate, 10X:
Composition per liter:
NaCl.........................................................6.8g
D- Glucose...................................................1.0g
KCl..........................................................0.4g
L- Cysteine·HCl·H2O.......................................0.26g
CaCl2 , anhydrous...........................................0.2g
MgSO4 ·7H2O................................................0.2g
NaH2PO4 ·H2O..............................................0.14g
Sodium acetate·3H2O......................................0.083g
L- Glutamic acid..........................................0.075g
L- Arginine·HCl...........................................0.07g
L- Lysine·HCl.............................................0.07g
L- Leucine ................................................0.06g
Glycine....................................................0.05g
Ascorbic acid..............................................0.05g
L- Proline.................................................0.04g
L- Tyrosine ...............................................0.04g
L- Aspartic acid...........................................0.03g
L- Threonine...............................................0.03g
L- Alanine................................................0.025g
L- Phenylalanine..........................................0.025g
L- Serine.................................................0.025g
L- Valine.................................................0.025g
L- Cystine.................................................0.02g
L- Histidine·HCl·H2O.....................................0.02g
L- Isoleucine..............................................0.02g
Phenol red.................................................0.02g
L- Methionine.............................................0.015g
Deoxyadenosine.............................................0.01g
Deoxycytidine..............................................0.01g
Deoxyguanosine ............................................0.01g
Glutathione, reduced.......................................0.01g
Thymidine..................................................0.01g
Hydroxy- L- proline .......................................0.01g
L- Tryptophan..............................................0.01g
Nicotinamide adenine dinucleotide .........................7.0mg
Tween ™ 80.................................................5.0mg
Sodium glucoronate·H2O....................................4.2mg
Coenzyme A ................................................2.5mg
Cocarboxylase..............................................1.0mg
Flavin adenine dinucleotide................................1.0mg
Nicotinamide adenine
dinucleotide phosphate.....................................1.0mg
Uridine triphosphate ......................................1.0mg
Choline chloride...........................................0.5mg
Cholesterol................................................0.2mg
5-Methyldeoxycytidine......................................0.1mg
Inositol..................................................0.05mg
p-Aminobenzoic acid.......................................0.05mg
Niacin...................................................0.025mg
Niacinamide..............................................0.025mg
Pyridoxine...............................................0.025mg
Pyridoxal·HCl...........................................0.025mg
Biotin....................................................0.01mg
D- Calcium pantothenate...................................0.01mg
Folic acid ...............................................0.01mg
Riboflavin ...............................................0.01mg
Thiamine·HCl ............................................0.01mg
pH 7.2 ± 0.2 at 25°C
Preparation of CMRL 1066 Medium without Glutamine,
without Bicarbonate, 10X: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Fil-
ter sterilize.
Preparation of Medium:  Add components, except gelatin solution
and rabbit serum, to 628.0mL of glass-distilled water. Mix thoroughly.
Adjust pH to 7.6–7.65. Add 200.0mL of 7% aqueous gelatin solution.
Filter sterilize entire medium. Aseptically add 72.0mL of sterile rabbit
serum.
Use: For the cultivation of a wide variety of microorganisms in a
chemically defined medium. For the cultivation of Borrelia and Spiro-
chaeta species.