BSK Medium, Modified

[所属分类:培养基配方] [发布时间:2020-3-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BSK Medium, Modified

培养基配方
Composition per 1264.0mL:
Bovine serum albumin, fraction V...............................50.0g
HEPES (N-[2-hydroxymethyl]piperazine-N′
[ethane sulfonate]) buffer.....................................6.0g
Neopeptone.....................................................5.0g
Glucose .......................................................5.0g
Yeastolate....................................................2.54g
NaHCO3 .......................................................2.2g
Sodium pyruvate...............................................0.8g
Sodium citrate................................................0.7g
MgSO4 ·7H2O..................................................0.6g
N-Acetylglucosamine...........................................0.4g
CaCl2 ·2H2O.................................................0.07g
CMRL 1066, 10X
without glutamine or NaHCO3 ..............................100.0mL
Rabbit serum, heat inactivated.............................64.0mL
pH 7.5 ± 0.2 at 25°C
CMRL 1066, 10X without Glutamine or NaHCO3 :
Composition per liter:
NaCl..........................................................6.8g
D- Glucose....................................................1.0g
KCl...........................................................0.4g
L- Cysteine·HCl·H2O........................................0.26g
CaCl2 , anhydrous.............................................0.2g
MgSO4 ·7H2O..................................................0.2g
NaH2PO4 ·H2O................................................0.14g
Sodium acetate·3H2O........................................0.083g
L- Glutamic acid............................................0.075g
L- Arginine·HCl............................................0.070g
L- Lysine·HCl..............................................0.070g
L- Leucine..................................................0.060g
Glycine.....................................................0.050g
Ascorbic acid ..............................................0.050g
L- Proline..................................................0.040g
L- Tyrosine.................................................0.040g
L- Aspartic acid ...........................................0.030g
L- Threonine................................................0.030g
L- Alanine..................................................0.025g
L- Phenylalanine............................................0.025g
L- Serine ..................................................0.025g
L- Valine ..................................................0.025g
L- Cystine .................................................0.020g
L- Histidine·HCl·H2O......................................0.020g
L- Isoleucine...............................................0.020g
Phenol Red..................................................0.020g
L- Methionine...............................................0.015g
Deoxyadenosine..............................................0.010g
Deoxycytidine...............................................0.010g
Deoxyguanosine .............................................0.010g
Glutathione, reduced........................................0.010g
Thymidine...................................................0.010g
Hydroxy- L- proline ........................................0.010g
L- Tryptophan...............................................0.010g
Nicotinamide adenine dinucleotide ..........................7.0mg
Tween ™ 80..................................................5.0mg
Sodium glucoronate·H2O.....................................4.2mg
Coenzyme A..................................................2.5mg
Cocarboxylase...............................................1.0mg
Flavin adenine dinucleotide.................................1.0mg
Nicotinamide adenine dinucleotide phosphate.................1.0mg
Uridine triphosphate .......................................1.0mg
Choline chloride...........................................0.50mg
Cholesterol................................................0.20mg
5-Methyldeoxycytidine......................................0.10mg
Inositol...................................................0.05mg
p-Aminobenzoic acid........................................0.05mg
Niacin....................................................0.025mg
Niacinamide...............................................0.025mg
Pyridoxine................................................0.025mg
Pyridoxal·HCl............................................0.025mg
Biotin....................................................0.01mg
D- Calcium pantothenate...................................0.01mg
Folic acid ...............................................0.01mg
Riboflavin................................................0.01mg
Thiamine·HCl.............................................0.01mg
Preparation of CMRL 1066, 10X Without Glutamine or
NaHCO3 :  Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Filter sterilize.
Preparation of Medium: Add components, except CMRL 1066,
10X without glutamine or NaHCO 3 and rabbit serum, to distilled/de-
ionized water and bring volume to 1100.0mL. Mix thoroughly. Adjust
pH to 7.5 with NaOH. Filter sterilize. Aseptically add 100.0mL of ster-
ile CMRL 1066, 10X without glutamine or NaHCO 3 and 64.0mL of
sterile rabbit serum. Mix thoroughly. Aseptically distribute 10.0mL
volumes into sterile 16 × 125.0mm test tubes.
Use: For the cultivation of Borrelia afzelii, Borrelia burgdorferi, and
Borrelia gorinii.