BSK Medium, Revised

[所属分类:培养基配方] [发布时间:2020-3-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BSK Medium, Revised

培养基配方
Composition per 1164.0mL:
Bovine serum albumin fraction V..............................50.0g
HEPES (N-[2-hydroxyethyl]piperazine-N′-2-
ethanesulfonic acid) buffer..................................6.0g
Neopeptone...................................................5.0g
Glucose......................................................5.0g
TC-Yeastolate ...............................................2.54g
NaHCO3 ......................................................2.2g
Sodium pyruvate..............................................0.8g
Sodium citrate...............................................0.7g
N-Acetylglucosamine .........................................0.4g
CMRL 1066, without glutamine,
without bicarbonate, 10X..................................100.0mL
Rabbit serum..............................................64.0mL
pH 7.6–7.65 at 25°C
CMRL 1066 Medium without Glutamine, without Bicar-
bonate, 10X:
Composition per liter:
NaCl......................................................6.8g
D- Glucose................................................1.0g
KCl.......................................................0.4g
L- Cysteine·HCl·H2O....................................0.26g
CaCl2 , anhydrous........................................0.2g
MgSO4 ·7H2O.............................................0.2g
NaH2PO4 ·H2O...........................................0.14g
Sodium acetate·3H2O...................................0.083g
L- Glutamic acid.......................................0.075g
L- Arginine·HCl.......................................0.070g
L- Lysine·HCl.........................................0.070g
L- Leucine.............................................0.060g
Glycine................................................0.050g
Ascorbic acid .........................................0.050g
L- Proline.............................................0.040g
L- Tyrosine............................................0.040g
L- Aspartic acid ......................................0.030g
L- Threonine...........................................0.030g
L- Alanine.............................................0.025g
L- Phenylalanine.......................................0.025g
L- Serine .............................................0.025g
L- Valine .............................................0.025g
L- Cystine ............................................0.020g
L- Histidine·HCl·H2O.................................0.020g
L- Isoleucine..........................................0.020g
Phenol red.............................................0.020g
L- Methionine..........................................0.015g
Deoxyadenosine.........................................0.010g
Deoxycytidine..........................................0.010g
Deoxyguanosine.........................................0.010g
Glutathione, reduced...................................0.010g
Thymidine..............................................0.010g
Hydroxy- L- proline....................................0.010g
L- Tryptophan..........................................0.010g
Nicotinamide adenine dinucleotide .....................7.0mg
Tween™ 80..............................................5.0mg
Sodium glucoronate·H2O................................4.2mg
Coenzyme A ............................................2.5mg
Cocarboxylase..........................................1.0mg
Flavin adenine dinucleotide............................1.0mg
Nicotinamide adenine
dinucleotide phosphate.................................1.0mg
Uridine triphosphate ..................................1.0mg
Choline chloride......................................0.50mg
Cholesterol...........................................0.20mg
5-Methyldeoxycytidine.................................0.10mg
Inositol..............................................0.05mg
p-Aminobenzoic acid...................................0.05mg
Niacin...............................................0.025mg
Niacinamide..........................................0.025mg
Pyridoxine...........................................0.025mg
Pyridoxal·HCl.......................................0.025mg
Biotin ...............................................0.01mg
Calcium  DL -pantothenate.............................0.01mg
Folic acid ...........................................0.01mg
Riboflavin............................................0.01mg
Thiamine·HCl ........................................0.01mg
pH 7.2 ± 0.2 at 25°C
Preparation of CMRL 1066 Medium without Glutamine,
without Bicarbonate, 10X: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Fil-
ter sterilize.
Preparation of Medium:  Add components, except rabbit serum
and CMRL 1066, to 1.0L of glass-distilled/deionized water. Mix thor-
oughly. Adjust pH to 7.5 with NaOH. Filter sterilize. Aseptically add
100.0mL of sterile CMRL 1066 and 64.0mL of sterile rabbit serum.
Adjust final pH to 7.5–7.6. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Borrelia burgdorferi, Borrelia afzelii, Bor-
relia garinii, Borrelia anserina, and Borrelia japonica.