BSL for Corynebacterium (Buffered Soy Lactose for Corynebacterium)

[所属分类:培养基配方] [发布时间:2020-3-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BSL for Corynebacterium
(Buffered Soy Lactose for Corynebacterium)

培养基配方
Composition per liter:
Agar.............................................................15.0g
Papaic digest of soybean meal....................................10.0g
Na2HPO4 ..........................................................6.0g
KH2PO4 ...........................................................3.0g
NH4Cl ............................................................1.0g
MgSO4 ·7H2O......................................................0.2g
Lactose solution...............................................100.0mL
pH 6.8–7.2 at 25°C
Lactose Solution:
Composition per 100.0mL:
Lactose........................................................10.0g
Preparation of Lactose Solution: Add lactose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add components, except lactose solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Heat gently with frequent mixing. Adjust pH to 6.8–7.2.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile lactose solution. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Curtobacterium flaccum-
faciens.