BSR Medium

[所属分类:培养基配方] [发布时间:2020-3-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BSR Medium

培养基配方
Composition per liter:
Beef heart, solids from infusion..........................500.0g
Sorbitol ..................................................70.0g
Sucrose....................................................10.0g
Tryptose...................................................10.0g
NaCl........................................................5.0g
Fructose....................................................1.0g
Glucose.....................................................1.0g
Phenol Red.................................................0.02g
Horse serum..............................................100.0mL
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 100.0mL of horse serum. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of Spiroplasma citri.