BTB Teepol ® Agar

[所属分类:培养基配方] [发布时间:2020-3-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BTB Teepol ® Agar

培养基配方
Composition per liter:
NaCl...........................................................20.0g
Agar...........................................................15.0g
Peptone .......................................................10.0g
Sucrose........................................................10.0g
Beef extract....................................................5.0g
Bromthymol Blue.................................................0.08g
Teepol .........................................................2.0mL
pH 7.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Teepol may be substituted by 0.1mL
of Tergitol™ 7. Mix thoroughly. Gently heat and bring to boiling. Ad-
just pH to 7.8. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes.
Use: For the isolation and cultivation of Vibrio anguillarum.