BTU Medium

[所属分类:培养基配方] [发布时间:2020-3-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BTU Medium

培养基配方
Composition per liter:
Ground meat, fat free.................................500.0g
Pancreatic digest of casein...........................30.0g
K2HPO4 ................................................5.0g
Yeast extract..........................................5.0g
L -Cysteine·HCl ......................................0.5g
Resazurin ............................................1.0mg
NaOH (1N solution)....................................25.0mL
Formate-fumarate solution.............................4.26mL
pH 7.0 ± 0.2 at 25°C
Formate-Fumarate Solution:
Composition per 100.0mL:
Sodium formate .............................................6.0g
Sodium fumarate.............................................6.0g
Preparation of Formate-Fumarate Solution : Add components
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Use lean beef or horse meat. Remove fat
and connective tissue. Grind finely. Add ground meat and 25.0mL of
NaOH solution to distilled/deionized water and bring volume to
1025.0mL. Gently heat and bring to boiling. Continue boiling for 15
min. without stirring. Cool to room temperature. Remove fat from sur-
face. Filter and retain both meat particles and filtrate. Adjust volume of
filtrate to 1.0L with distilled/deionized water. Add pancreatic digest of
casein, K2HPO4 , yeast extract, and resazurin. Gently heat and bring to
boiling. Boil for 1–2 min. Add  L -cysteine·HCl. Mix thoroughly. Dis-
tribute 7.0mL into tubes that contain meat particles (1 part meat parti-
cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C.
Prior to inoculation, add 30.0μL of formate-fumarate solution for each
milliliter of medium in the tubes.
Use: For the cultivation of Bacteroides ureolyticus.