Buffered Clostridial Medium with Cellobiose

[所属分类:培养基配方] [发布时间:2020-3-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Buffered Clostridial Medium with Cellobiose

培养基配方
Composition per liter:
Meat extract..................................................10.0g
Peptone ......................................................10.0g
Cellobiose.....................................................5.0g
Glucose........................................................5.0g
NaCl...........................................................5.0g
Sodium acetate.................................................3.0g
Yeast extract..................................................3.0g
NaHCO3 .......................................................2.75g
Soluble starch.................................................1.0g
L -Cysteine·HCl·H2O..........................................0.5g
Resazurin.....................................................1.0mg
Hemin solution...............................................10.0mL
Vitamin K 1 solution..........................................0.2mL
pH 7.0 ± 0.2 at 25°C
Hemin Solution:
Composition per 100.0mL:
Hemin .......................................................50.0mg
NaOH (1N solution)...........................................1.0mL
Preparation of Hemin Solution: Dissolve hemin in 1.0mL of 1N
NaOH solution. Bring volume to 100.0mL with distilled/deionized wa-
ter. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Vitamin K 1 Solution:
Composition per 30.15mL:
Ethanol (95% solution)................................................30.0mL
Vitamin K 1 ..........................................................0.15mL
Preparation of Vitamin K 1 Solution: Combine components. Mix
thoroughly. Store at 4°C in the dark. Discard solution after 1 month.
Preparation of Medium: Prepare and dispense medium under 80%
N2 + 10% CO2  + 10% H2 . Add components, except cellobiose,
NaHCO3 , and  L -cysteine·HCl·H2O, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Continue boiling for 3 min. Cool to room temperature while sparg-
ing with 80% N2 + 10% CO2  + 10% H2 . Add cellobiose, NaHCO3 , and
L -cysteine·HCl·H2O, in that order. Mix thoroughly. Adjust pH to 7.0.
Anaerobically distribute into tubes. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the cultivation of Eubacterium xylanophilum and Clostrid-
ium termitidis.