Buffered Enrichment Broth (BAM M52)

[所属分类:培养基配方] [发布时间:2020-3-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Buffered Enrichment Broth
(BAM M52)

培养基配方
Composition per liter :
Na2HPO4 .......................................................9.6g
KH2PO4 .......................................................1.35g
Pyruvate solution ...........................................11.1mL
Nalidixic acid solution.......................................8.0mL
Cycloheximide solution........................................5.0mL
Acriflavin solution...........................................2.0mL
pH 7.3 ± 0.1 at 25°C
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid, sodium salt...................................0.05g
Preparation of Nalidixic Acid Solution: Add nalidixic acid to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Acriflavin Solution:
Composition per 10.0mL:
Acriflavin·HCl .............................................0.05g
Preparation of Acriflavin Solution: Add acriflavin·HCl to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide..............................................0.1g
Ethanol, 40%.............................................10.0mL
Preparation of Cycloheximide Solution: Add cycloheximide to
40% ethanol and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Pyruvate Solution:
Composition per 20.0mL:
Na-pyruvate ................................................2.0g
Preparation of Pyruvate Solution: Add Na-pyruvate to distilled/
deionized water and bring volume to 20.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except pyruvate solu-
tion, nalidixic acid solution, acriflavin solution, and cycloheximide so-
lution, to distilled/deionized water and bring volume to 973.9.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C. Aseptically add 11.1mL sterile
pyruvate solution. Mix thoroughly. Aseptically add 8.0mL sterile nali-
dixic acid solution, 5.0mL sterile cycloheximide solution, and 2.0mL
sterile acriflavin solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use : For the cultivation of of Listeria spp.