Buffered Listeria Enrichment Broth Base with Listeria Selective Supplement

[所属分类:培养基配方] [发布时间:2020-3-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Buffered Listeria Enrichment Broth Base
with Listeria Selective Supplement

培养基配方
Composition per liter:
Casein enzymic hydrolysate......................................17.0g
Na2HPO4 .........................................................9.6g
Yeast extract....................................................6.0g
NaCl.............................................................5.0g
Papaic digest of soybean meal....................................3.0g
K2HPO4 ..........................................................2.5g
Glucose .........................................................2.5g
KH2PO4 .........................................................1.35g
Sodium pyruvate..................................................1.0g
Selective supplement solution .................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Cycloheximide....................................................25.0g
Acriflavin hydrochloride.........................................5.0mg
Nalidixic acid...................................................5.0mg
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the enrichment and cultivation Listeria monocytogenes.