Buffered Marine Yeast Medium

[所属分类:培养基配方] [发布时间:2020-3-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Buffered Marine Yeast Medium

培养基配方
Composition per liter:
NaCl.................................................................24.0g
Agar ................................................................20.0g
Yeast extract.........................................................5.0g
1M Phosphate buffer, pH 6.8.........................................20.0mL
Hutner’s mineral base..............................................20.0mL
KOH (1N) ............................................................7.0mL
pH 6.8 ± 0.2 at 25°C
1M Phosphate Buffer, pH 6.8:
Composition per liter:
K2H2PO4 ..............................................................85.4g
NaH2PO4 ·H2O.........................................................70.4g
Preparation of 1M Phosphate Buffer, pH 6.8: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 6.8.
Hutner’s Mineral Base:
Composition per liter:
MgSO4· 7H2O ........................................................29.7g
Nitrilotriacetic acid................................................10.0g
CaCl2 ·2H2O.........................................................3.34g
FeSO4 ·7H2O.........................................................0.01g
(NH4)2 MoO4 .......................................................9.25mg
Metals “44”......................................................50.0mL
Preparation of Hutner’s Mineral Base: Initially add a few drops of
H2SO4 to the distilled water to retard precipitation. Dissolve the nitrilotri-
acetic acid first and neutralize the solution with KOH. Add the other ingre-
dients and adjust the pH to 7.2 with KOH and/or H 2 SO 4 . There may be a
slight precipitate. Store at 5°C.
Metals “44”:
Composition per 100.0mL:
ZnSO4 ·7H2O.......................................................1.1g
FeSO4 ·7H2O.......................................................0.5g
EDTA.............................................................0.25g
MnSO4 ·7H2O....................................................0.154g
CuSO4 ·5H2O.....................................................0.04g
Co(NO3)2 ·6H2O.................................................0.025g
Na2B4O7 ·10H2O.................................................0.018g
Preparation of Metals “44”:  Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Add aseptically to sterile basal
medium.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Pseudomonas species.