Burke’s Modified Nitrogen-Free Medium

[所属分类:培养基配方] [发布时间:2020-3-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Burke’s Modified Nitrogen-Free Medium

培养基配方
Composition per liter:
MgSO4 ·7H2O......................................................0.2g
Na2HPO4 .........................................................0.19g
NaHCO3 ..........................................................0.05g
CaSO4 ·2H2O.....................................................0.02g
KH2PO4 .........................................................0.011g
SrCl2 ·6H2O ....................................................0.01g
NaCl.............................................................0.01g
Adenine.........................................................0.01g
FeSO4· 7H2O....................................................6.0mg
Na2MoO3 ........................................................0.5mg
pH 7.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Azotobacter vinelandii.