Burke’s Modified Nitrogen-Free Medium

[所属分类:培养基配方] [发布时间:2020-3-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Burke’s Modified Nitrogen-Free Medium

培养基配方
Composition per liter:
Noble agar.....................................................15.0g
Glucose........................................................10.0g
Cellulose......................................................10.0g
K2HPO4 .........................................................1.0g
CaCl2 ·2H2O ...................................................0.1g
MgSO4 ·7H2O...................................................0.02g
FeSO4 ·7H2O..................................................50.0mg
Na2MoO4 ·2H2O................................................25.0mg
Vitamin B 12 ..................................................0.1mg
Vitamin solution...............................................1.0mL
pH 7.2–7.3 ± 0.2 at 25°C
Vitamin Solution:
Composition per 50.0mL:
Thiamine·HCl ...............................................843.3mg
Pantothenic acid.............................................595.8mg
Nicotinic acid...............................................307.8mg
p-Aminobenzoic acid...........................................68.6mg
Pyridoxamine·2HCl............................................60.3mg
Biotin........................................................50.0mg
Folic acid ...................................................11.0mg
Vitamin B 12 ..................................................3.5mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 50.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except glucose, cellu-
lose, K2HPO4 , and vitamin solution, to distilled/deionized water and
bring volume to 850.0mL. Mix thoroughly. Adjust pH to 7.2–7.3. In
three separate flasks, add glucose, cellulose, and K 2 HPO 4 to 50.0mL of
distilled/deionized water. Filter sterilize the vitamin solution. Auto-
clave the other solutions separately for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically combine all the solutions and mix
thoroughly. Distribute into sterile tubes or flasks or pour into sterile Pe-
tri dishes.
Use: For the cultivation and maintenance of Streptomyces species.