Burkholderia cepacia Agar

[所属分类:培养基配方] [发布时间:2020-3-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Burkholderia cepacia Agar

培养基配方
Composition per liter:
Agar .........................................................12.0g
Sodium pyruvate................................................7.0g
Peptone........................................................5.0g
KH2PO4 ........................................................4.4g
Yeast extract..................................................4.0g
Bile salts.....................................................1.5g
Na2HPO4 .......................................................1.4g
(NH4)2SO4 .....................................................1.0g
MgSO4 ..........................................................0.2
Phenol Red....................................................0.02g
Fe(NH4)2(SO4)2 ·6H2O ........................................0.01g
Crystal Violet...............................................0.001g
Selective supplement solution ...............................10.0mL
pH 6.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Selective Supplement Solution:
Composition per 10.0mL:
Polymyxin B.............................................150,000IU
Ticarcillin..............................................100.0mg
Gentamicin.................................................5.0mg
Preparation of Selective Supplement Solution:  Add components
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat while stirring and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptially add 10.0mL selective supplement solution. Mix thoroughly.
Pour into sterile Petri dishes.
Use:  For the selective isolation of Burkholderia cepacia from the
respiratory secretions of patients with cystic fibrosis and for routine
testing of non-sterile inorganic salt solutions containing preservative.
Slow growing B. cepacia can be missed on conventional media such as
blood or MacConkey agar due to overgrowth caused by other faster
growing organisms found in the respiratory tract of CF patients such as
mucoid Klebsiella species, Pseudomonas aeruginosa, and Staphylo-
coccus species. This may lead to the infection being missed or wrongly
diagnosed.