Burkholderia pseudomallei Selective Agar (BPSA)

[所属分类:培养基配方] [发布时间:2020-3-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Burkholderia pseudomallei Selective Agar
(BPSA)

培养基配方
Composition per liter:
Agar..............................................................15.0g
Pancreatic Digest of Casein .......................................5.0g
Maltose ...........................................................4.0g
Yeast Extract .....................................................2.5g
Glucose............................................................1.0g
Neutral Red........................................................0.1g
Gentamicin solution..............................................10.0mL
Glycerol ........................................................10.0mL
Nile Blue solution................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Gentamicin Solution:
Composition per 10.0mL:
Gentamicin.....................................................20.0mg
Preparation of Gentamicin Solution:  Add gentamicin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Nile Blue Solution:
Composition per 10.0mL:
Nile Blue........................................................0.2g
Preparation of Nile Blue Solution:  Add Nile blue to 10.0mL of a
1% solution of dimethyl sulfoxide. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except gentamcin solu-
tion, Nile Blue solution, and glycerol, to distilled/deionized water and
bring volume to 979.0mL. Mix thoroughly. Gently heat while stirring
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°C. Aseptially add 10.0mL sterile gentamicin solution,
1.0mL sterile Nile blue solution, and 10.0mL filter-sterilized glycerol.
Mix thoroughly for 5 min on a heated magnetic stirrer at 40°C. Pour
into sterile Petri dishes.
Use:  For the cultivation of Burkholderia pseudomallei from clinical
specimens collected from non-sterile sites with improved recovery of
the more easily inhibited strains of B. pseudomallei.