Burk’s Medium

[所属分类:培养基配方] [发布时间:2020-3-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Burk’s Medium

培养基配方
Composition per liter:
Sucrose...........................................................20.0g
MgSO4 ·7H2O.......................................................0.2g
K2HPO4 ............................................................0.8g
KH2PO4 ...........................................................0.25g
CaSO4 ............................................................0.13g
FeCl3 ...........................................................1.45mg
Na2MoO3 ........................................................0.253mg
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For thecultivation of nitrogen fixing bacteria, such as Azotrobacter
spp., from soil.