Bushnell-Haas Agar

[所属分类:培养基配方] [发布时间:2020-3-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bushnell-Haas Agar

培养基配方
Composition per liter:
Agar ............................................................15.0g
KH2PO4 ...........................................................1.0g
K2HPO4 ...........................................................1.0g
NH4NO3 ...........................................................1.0g
MgSO4 ·7H2O......................................................0.2g
FeCl3 ...........................................................0.05g
CaCl2 ·2H2O.....................................................0.02g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. For
use in cultivating hydrocarbon-utilizing bacteria, layer 0.1–1.0% hy-
drocarbon on agar surface or aseptically add sterile hydrocarbon to
cooled agar prior to pouring plates.
Use: For examining fuels for microbial contamination and for study-
ing hydrocarbon utilization by microorganisms. Also for the cultiva-
tion of Nocardia species.