Bushnell-Haas Broth

[所属分类:培养基配方] [发布时间:2020-3-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bushnell-Haas Broth

培养基配方
Composition per liter:
KH2PO4 .............................................................1.0g
K2HPO4 .............................................................1.0g
NH4NO3 .............................................................1.0g
MgSO4 ·7H2O........................................................0.2g
FeCl3 .............................................................0.05g
CaCl2 ·2H2O.......................................................0.02g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. For use in
cultivating hydrocarbon-utilizing bacteria, layer 0.1–1.0% hydrocar-
bon on broth surface or add directly to broth.
Use: For examining fuels for microbial contamination and for studying
the hydrocarbon utilization by microorganisms. Also for the cultivation of
Nocardia species.