Caffeic Acid Ferric Citrate Test Medium (CAFC Test Medium) (Caffeic Acid Agar)

[所属分类:培养基配方] [发布时间:2020-3-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Caffeic Acid Ferric Citrate Test Medium
(CAFC Test Medium)
(Caffeic Acid Agar)
Composition per liter:
Agar .........................................................20.0g
(NH4)2SO4 ....................................................5.0g
Glucose ......................................................5.0g
Yeast extract.................................................2.0g
K2HPO4 .......................................................0.8g
MgSO4 ·3H2O..................................................0.7g
Caffeic acid· 1/2H2O .......................................0.18g
Chloramphenicol..............................................0.05g
Ferric citrate solution......................................4.0mL
pH 6.5 ± 0.2 at 25°C
Ferric Citrate Solution:
Composition per 20.0mL:
Ferric citrate.............................................100.0mg
Preparation of Ferric Citrate Solution: Add ferric citrate to
20.0mL of distilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components, except chlorampheni-
col, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Heat to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 0.05g of chloramphenicol.
Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and presumptive identification of Cryptococcus
neoformans. Cryptococcus neoformans appears as dark brown colo-
nies. All other Cryptococcus species appear as light brown or nonpig-
mented colonies.