Caffeine Medium

[所属分类:培养基配方] [发布时间:2020-3-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Caffeine Medium

培养基配方
Composition per liter:
Agar ............................................................15.0g
Solution A.....................................................400.0mL
Solution B.....................................................400.0mL
Solution C.....................................................200.0mL
pH 5.0 ± 0.2 at 25°C
Solution A:
Composition per 400.0mL:
Na2HPO4 .......................................................7.8g
KH2PO4 ........................................................3.0g
Caffeine.......................................................1.0g
NaCl..........................................................0.58g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 5.0.
Solution B:
Composition per 400.0mL:
MgSO4 ·7H2O...................................................0.12g
CaCl2 ·2H2O .................................................11.0mg
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly.
Solution C:
Composition per 200.0mL:
FeCl3 ........................................................16.0mg
Preparation of Solution C:  Add FeCl3 to distilled/deionized water
and bring volume to 200.0mL. Mix thoroughly.
Preparation of Medium:  To 400.0mL of solution A, add 400.0mL
of solution B and 200.0mL of solution C. Adjust pH to 5.0. Add agar.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation of Pseudomonas species.