Caldicellulosiruptor Medium

[所属分类:培养基配方] [发布时间:2020-3-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Caldicellulosiruptor Medium

培养基配方
Composition per liter:
Pancreatic digest of casein.....................................2.0g
K2HPO4 .........................................................1.5g
Cellobiose......................................................1.0g
Yeast extract...................................................1.0g
NaCl............................................................0.9g
NH4Cl ..........................................................0.9g
KH2PO4 ........................................................0.75g
L -Cysteine·HCl ..............................................0.75g
MgCl2 ·6H2O ...................................................0.4g
FeCl3 ·6H2O...................................................2.5mg
Resazurin .....................................................0.5mg
Trace elements solution SL-10..................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O....................................................1.5g
CoCl2 ·6H2O.................................................190.0mg
MnCl2 ·4H2O ................................................100.0mg
ZnCl2 ........................................................70.0mg
Na2MoO4 ·2H2O ...............................................36.0mg
NiCl2 ·6H2O..................................................24.0mg
H3BO3 .........................................................6.0mg
CuCl2 ·2H2O ..................................................2.0mg
HCl (25% solution)............................................10.0mL
Preparation of Trace Elements Solution SL-10: Add
FeCl2 ·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/
deionized water and bring volume to 1.0L. Add remaining compo-
nents. Mix thoroughly. Sparge with 100% N2 . Autoclave for 15 min at
15 psi pressure–121°C.
Preparation of Medium:  Prepare and dispense medium under
100% N2 . Add components to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Sparge with 100% N2 . Anaerobically
distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Caldicellulosiruptor saccharolyticus.