Caldicellulosiruptor Medium

[所属分类:培养基配方] [发布时间:2020-3-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Caldicellulosiruptor Medium

培养基配方
Composition per 1001.0mL:
Pancreatic digest of casein.......................................2.0g
K2HPO4 ...........................................................1.5g
Cellulose ........................................................1.0g
Cellobiose .......................................................1.0g
Yeast extract.....................................................1.0g
NaCl..............................................................0.9g
NH4Cl ............................................................0.9g
KH2PO4 ..........................................................0.75g
MgCl2 ·6H2O......................................................0.4g
FeCl3 ·6H2O.....................................................2.5mg
L -Cysteine·HCl.................................................0.75g
Resazurin .......................................................0.5mg
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O......................................................1.5g
CoCl2 ·6H2O ..................................................190.0mg
MnCl2 ·4H2O...................................................100.0mg
ZnCl2 ..........................................................70.0mg
Na2MoO4 ·2H2O .................................................36.0mg
NiCl2 ·6H2O....................................................24.0mg
H3BO3 ...........................................................6.0mg
CuCl2 ·2H2O ....................................................2.0mg
HCl (25% solution)..............................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium:  Prepare and dispense medium under
100% N2 . Add components to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Sparge with 100% N2 . Anaerobically
distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Caldicellulosiruptor saccharolyticus.