Campylobacter Agar with 5 Antimicrobics and 10% Sheep Blood

[所属分类:培养基配方] [发布时间:2020-3-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Agar
with 5 Antimicrobics and 10% Sheep Blood

培养基配方
Composition per liter:
Agar ............................................................15.0g
Pancreatic digest of casein......................................10.0g
Peptic digest of animal tissue...................................10.0g
NaCl..............................................................5.0g
Yeast extract.....................................................2.0g
Glucose ..........................................................1.0g
NaHSO3 ...........................................................0.1g
Sheep blood, defibrinated......................................100.0mL
Antibiotic supplement...........................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Antibiotic Supplement:
Composition per 10.0mL:
Cephalothin....................................................0.015g
Vancomycin......................................................0.01g
Trimethoprim....................................................5.0mg
Amphotericin B .................................................2.0mg
Polymyxin B.....................................................2500U
Preparation of Antibiotic Supplement: Add components to
10.0mL of distilled/deionized water. Filter sterilize.
Preparation of Medium: Add components, except sheep blood and
antibiotic solution, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 100.0mL of sterile sheep blood and 10.0mL of sterile antibiotic so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the primary selective isolation and cultivation of Campylobacter
jejuni from human fecal specimens.