Campylobacter Agar, Blaser’s (Blaser’s Campylobacter Agar)

[所属分类:培养基配方] [发布时间:2020-3-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Agar, Blaser’s
(Blaser’s Campylobacter Agar)

培养基配方

Composition per liter:
Campylobacter agar base......................................990.0mL
Supplement B..................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Campylobacter Agar Base:
Composition per liter:
Proteose peptone...............................................15.0g
Agar...........................................................12.0g
NaCl............................................................5.0g
Yeast extract...................................................5.0g
Liver digest....................................................2.5g
Source: Campylobacter agar base and Campylobacter antimicrobic
supplement B are available as a premixed powder from BD Diagnostic
Systems.
Preparation of Campylobacter Agar Base: Add components to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C.
Supplement B:
Composition per 10.0mL:
Cephalothin...................................................15.0mg
Vancomycin....................................................10.0mg
Trimethoprim...................................................5.0mg
Amphotericin B ................................................2.0mg
Polymyxin B....................................................2500U
Preparation of Supplement B:  Add components to 10.0mL of dis-
tilled/deionized water. Filter sterilize.
Preparation of Medium: Prepare 990.0mL of Campylobacter agar
base. Autoclave and cool to 45°–50°C. Aseptically add 10.0mL of sterile
supplement B. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation of Campylobacter jejuni from fecal
specimens, food, and environmental specimens.