Campylobacter Blood-Free Agar Base, Modified (CCDA, Modified)

[所属分类:培养基配方] [发布时间:2020-3-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Blood-Free Agar Base, Modified
(CCDA, Modified)

培养基配方
Composition per liter:
Peptone...........................................................20.0g
Agar .............................................................12.0g
NaCl...............................................................5.0g
Activated charcoal ................................................4.0g
Casein hydrolysate.................................................3.0g
Na-desoxycholate...................................................1.0g
Na-pyruvate ......................................................0.25g
FeSO4 ............................................................0.25g
Cefoperazone-amphotericin B solution.............................10.0mL
pH 7.4 ± 0.2 at 25°C
Cefoperazone-Amphotericin B Solution:
Composition per 10.0mL:
Cefoperazone.....................................................0.016g
Amphotericn B....................................................0.005g
Preparation of Cefoperazone-Amphotericin B Solution: Add
cefoperazone and amphotericin B to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components except cefoperazone-
amphotericin B solution to distilled/deionized water and bring volume
to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 10.0mL of cefoperazone-
amphotericin B solution. Mix thoroughly. Pour into sterile Petri dishes
or aseptically distribute into tubes or flasks.
Use: For the isolation of Campylobacter spp. from foods.The use of
Campylobacter Blood-Free Selective Agar is specified by the UK Min-
istry of Agriculture, Fisheries and Food (MAFF) in a validated method
for isolation of Campylobacter from foods. Amphotericin largely
reduces the growth of yeasts and molds. Cefoperazone especially
inhibits Enterobacteriaceae.