Campylobacter Blood-Free Selective Agar

[所属分类:培养基配方] [发布时间:2020-3-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Blood-Free Selective Agar

培养基配方
Composition per liter:
Agar.......................................................12.0g
Beef extract...............................................10.0g
Peptone ...................................................10.0g
Charcoal....................................................4.0g
Casein hydrolysate..........................................3.0g
Sodium deoxycholate.........................................1.0g
Fe2SO4 ·H2O...............................................0.25g
Sodium pyruvate............................................0.25g
Cefoperazone solution.....................................10.0mL
pH 7.4 ± 0.2 at 25°C
Cefoperazone Solution:
Composition per 10.0mL:
Sodium cefoperazone........................................0.032g
Preparation of Cefoperazone Solution: Add sodium cefopera-
zone to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except cefoperazone
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Heat with frequent agitation and boil for 1 min to
completely dissolve. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°–55°C. Add 10.0mL of sterile cefoperazone solution. Ad-
dition of 10.0mg/L of amphotericin B improves the selectivity of the
medium. Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the selective isolation of Campylobacter species, especially
Campylobacter jejuni, Campylobacter coli, and Campylobacter
laridis.