Campylobacter Charcoal Differential Agar (CCDA) (Preston Blood-Free Medium)

[所属分类:培养基配方] [发布时间:2020-3-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Charcoal Differential Agar
(CCDA)
(Preston Blood-Free Medium)
Composition per liter:
Agar.............................................................12.0g
Beef extract.....................................................10.0g
Peptone .........................................................10.0g
NaCl..............................................................5.0g
Charcoal..........................................................4.0g
Casein hydrolysate................................................3.0g
Sodium deoxycholate...............................................1.0g
FeSO4 ...........................................................0.25g
Sodium pyruvate..................................................0.25g
Cefoperazone solution...........................................10.0mL
pH 7.5 ± 0.2 at 25°C
Cefoperazone Solution:
Composition per 10.0mL:
Sodium cefoperazone.............................................0.032g
Preparation of Cefoperazone Solution: Add sodium cefopera-
zone to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except cefoperazone
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL
of sterile cefoperazone solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of Campylobacter species.