Campylobacter Enrichment Broth

[所属分类:培养基配方] [发布时间:2020-3-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Enrichment Broth

培养基配方
Composition per liter:
Beef extract....................................................10.0g
Peptone.........................................................10.0g
Yeast extract....................................................6.0g
NaCl.............................................................5.0g
Horse blood, laked.............................................50.0mL
FBP solution ...................................................4.0mL
Antibiotic solution ............................................4.0mL
pH 7.5 ± 0.2 at 25°C
Horse Blood, Laked:
Composition per 50.0mL:
Horse blood, fresh.............................................50.0mL
Preparation of Horse Blood, Laked: Add blood to a sterile poly-
propylene bottle. Freeze overnight at −20°C. Thaw at 8°C. Refreeze at
−20°C. Thaw again at 8°C.
FBP Solution:
Composition per 100.0mL:
FeSO4 ...........................................................6.25g
NaHSO3 ..........................................................6.25g
Sodium pyruvate..................................................6.25g
Preparation of FBP Solution: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide.................................................0.1g
Sodium cefoperazone..........................................0.03g
Trimethoprim lactate.......................................0.0125g
Rifampicin ..................................................0.01g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components—except laked horse
blood, FBP solution, and antibiotic solution—to distilled/deionized water
and bring volume to 942.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of sterile laked horse blood, 4.0mL of FBP
solution, and 4.0mL of antibiotic solution. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Campylobacter species from
dairy products.