Campylobacter Enrichment HiVeg Broth Base with Blood and Antibiotics (Preston Enrichment HiVeg Broth Base)

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Enrichment HiVeg Broth Base
with Blood and Antibiotics

培养基配方
(Preston Enrichment HiVeg Broth Base)
Composition per liter:
Plant extract ...................................................10.0g
Plant peptone....................................................10.0g
NaCl..............................................................5.0g
Horse blood, lysed .............................................50.0mL
Antibiotic supplement...........................................10.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium, without horse blood and antibiotic supplement,
is available as a premixed powder from HiMedia.
Antibiotic Supplement:
Composition per 10.0mL:
Cycloheximide......................................................0.1g
Rifampicin .......................................................0.01g
Trimethoprim lactate..............................................0.01g
Polmyxin B........................................................5000U
Preparation of Antibiotic Supplement: Add components to
10.0mL of 50:50 acetone:distilled/deionized water. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except horse blood and
antibiotic supplement, to distilled/deionized water and bring volume to
940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 50.0mL of lysed horse blood and 10.0mL of sterile antibiotic sup-
plement. Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the selective isolation of Campylobacter species.