Campylobacter fecalis Medium

[所属分类:培养基配方] [发布时间:2020-3-31] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter fecalis Medium

培养基配方
Composition per 1133.6mL:
Starch, soluble.............................................2.4g
Yeast extract...............................................2.4g
Blood agar base.............................................1.0L
Bovine blood.............................................120.0mL
Antibiotic solution.......................................10.0mL
Sodium lactate (60% syrup).................................3.6mL
pH 6.8 ± 0.2 at 25°C
Blood Agar Base:
Composition per liter:
Beef heart, solids from infusion..........................500.0g
Agar.......................................................15.0g
Tryptose...................................................10.0g
NaCl........................................................5.0g
Preparation of Blood Agar Base: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Gently
heat while stirring and bring to boiling.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide...............................................0.12g
Albamycin...................................................6.0mg
Bacitracin..................................................6000U
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium:  To 1.0L of blood agar base, add soluble
starch, yeast extract, and sodium lactate. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile bovine blood and antibiotic solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and isolation of Campylobacter fecalis.